Impact of exposure of larvae to boscalid at field concentrations on gene expression in honey bees
Résumé
Apis mellifera often encounters the fungicide boscalid in agricultural landscape. In this study, we assessed the impacts of ingesting boscalid and Pictor Pro® (a commercial formulation of boscalid) at envi- ronmentally relevant doses during the larval development phase. Following chronic exposure, we measured survival, development time, and gene expression in both larvae and in 10-day-old workers. The genes analyzed were involved in immunity, detoxification, development, and mitochondrial activity pathways. We found no sig- nificant impact of boscalid in larval survival and emergence. However, genes related to cytochrome b, succinate dehydrogenase, and catalase were downregulated in larvae and adult workers exposed to boscalid, though the differences were not significant when compared to the solvent control. Late variation was observed in 10 days adult bees’ overexpression of the vitellogenin gene, which is linked to development. The effects observed with boscalid molecule were not seen with the commercially formulated product, Pictor Pro®.
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